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This research assessed the effects of the implementation of the Treasury Single Account (TSA) Policy at Imo State University, Owerri, Nigeria. The study investigated its impact on financial accountability, financial autonomy, and financial constraints experienced by theuniversity management. A survey research design was employed, with data collected via questionnaires from 314 randomly selected respondents. Data analysis utilized percentage and mean statistical methods. This study reveals several key findings regarding the impact of the TSA policy at Imo State University in Owerri. Firstly, the implementation of the TSA policy has significantly enhanced financial accountability within the institution. However, it has also adversely affected the university