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This study assessed the influence of depression, anxiety and stress on the gambling behaviour of young people in Makurdi Metropolis, Benue State, Nigeria. One hundred and thirty-seven participants were purposively sampled for this study. The Depression, Anxiety and Stress Scale (DASS-21) and Problem Gambling Severity Index (PGSI) were used for data collection. This study utilized a cross-sectional survey research design, and univariate analysis of variance was the statistical tool used for data analysis. Three hypotheses were tested, and the results revealed that there was a significant main effect of depression on gambling behaviour among youths in the Makurdi Metropolis [F (2,137) = 8.968, P<.01], with an effect size of 14.2., indicating high effect the predictor variable on depression. However, anxiety [F(3,137) = .775, P>.05] and stress [F(2,137) = .099, P>.05] had no significant main effects on gambling behaviour among the participants. Multiple comparisons of depression levels showed that youths with normal depression significantly differed from those with mild (7.6; p<.05), moderate (30.04; p<.05) or extremely severe depression (7.97; p<05) in terms of gambling behaviour. The results also revealed that the gambling behaviour of youths with mild depression significantly differed from that of youths with moderate depression (5.51; p<.05) but not those with extremely severe depression (.41; p>.05). Similarly, youth with moderate depression did not significantly differ from those with extremely severe depression (4.49; p>.05). Based on these findings, the researchers recommended urgent public health interventions for young people with depression as a way of minimizing problem gambling among the young people in the study area.