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This study investigated the perceived impacts of job stress and work locus of control on the psychological well-being among federal employed medical doctors and nurses in South-East Nigeria. Two hypotheses were postulated and tested from data received from three hundred and four participants who were selected through the convenience sampling technique. Participants comprised of 111 Medical Doctors and 193 Nurses (166 males, 138 females) with ages ranging from 30 to 58 years (M = 44.20; SD = 6.28). The participants were administered the Job Stress Scale (JSS) by Parker and Decottiis (1983), the Work Locus of Control Scale (WLCS) by Spector (1988), and the Psychological Well-being Scale (PWBS) by Ryff (1989). The cross-sectional survey design was adopted while the hierarchical multiple regression statistics was used to analyse the data collected. The study