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The study explored whether perceived organizational support and level of education influence motivation among nurses in public hospitals in the south eastern region of Nigeria despite the loss of their colleagues to Covid-19 virus. Data were collected using crosssectional design from a sample of 203(120females, 59.1% and 83 males, 40.9%) nurses in Imostate specialist hospital Umuguma, Enugu State University Teaching Hospital, Park Lane, Enugu and Nnamdi Azikiwe University Teaching Hospital Awka, Anambra state, all in South-Eastern part Nigeria. Short version of Perceived Organizational Support (SPOS) (Eisenberger, Huntington, Huchison, & Sowa, 1986) and Situational Motivation Scale (SIMS) (Guay, Vallerand, & Blanchard, 2000) were instruments used to elicit information. The hierarchical multiple regression results showed that level of education is not a significant correlate of motivation, Perceived Organizational Support positively predicts motivation (β = .46, p<.01). The findings of the study were discussed, conclusions were drawn and recommendations made.